Glycobiology Protocols (Methods in Molecular Biology, Vol 347)

A distinguished panel of international experts reviews the importance of glycobiology, an interdisciplinary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Glycobiology Protocols highlights important methodological progress that has been made in the field of glycobiology, helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycoconjugates, and on the functions of lipid- or protein-bound carbohydrates.
Glycobiology Protocols contains specific methods for the analysis of the structures or functions of glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans. The methods described for specific systems can be modified for investigations of similar biomolecules and tissues or cell types. Comprehensive and highly practical, Glycobiology Protocols provides researchers with a solid basis for studying the many aspects of glycobiology with state-of-the-art methods. The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, with each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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YAC Protocols 2nd ed (Methods in Molecular Biology, Vol 349)

This completely revised and updated edition of YAC Protocols takes advantage of the many new advances in biology since the publication of the first edition, including the sequencing of the human genome, the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease. This volume also provides a much-needed update on the new techniques currently employed and to help redefine and illustrate the important roles still to be played by yeast artificial chromosome (YAC) technologies in the postgenomic age.

YAC Protocols, Second Edition, demonstrates the enormous potential use of YAC technologies in understanding the role of genetics in maintaining health, promoting pathogenesis, and conferring susceptibility to disease. Offering solutions to the many problems that have plagued researchers, this new edition allows for the wider and more efficient use of YAC technologies for the analysis of genetic and pathogenic causes of human disease.

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Mass Spectometry Data Analysis in Proteomics (Methods in Molecular Biology, Vol 367)

Mass Spectrometry Data Analysis in Proteomics is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. As MS is a high throughput technique, proteomic researchers must attend carefully to the associated field of data analysis, and this volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters. Several different types of MS data are discussed, followed by a description of optimal methods for conversion of raw data into peak lists for input to search engines. Choosing the most accurate and complete databases is emphasized, and a report of available sequence databases is included. Methods for assembling expressed sequence tags (ESTs) into assembled nonredundant databases are provided, along with protocols for further processing the sequences into a format suitable for MS data. Mass Spectrometry Data Analysis in Proteomics describes publicly available applications whenever possible.

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Brain and Visual Perception: The Story of a 25-Year Collaboration

Scientists’ understanding of two central problems in neuroscience, psychology, and philosophy has been greatly influenced by the work of David Hubel and Torsten Wiesel: (1) What is it to see? This relates to the machinery that underlies visual perception. (2) How do we acquire the brain’s mechanisms for vision? This is the nature-nurture question as to whether the nerve connections responsible for vision are innate or whether they develop through experience in the early life of an animal or human. This is a book about the collaboration between Hubel and Wiesel, which began in 1958, lasted until about 1982, and led to a Nobel Prize in 1981. It opens with short autobiographies of both men, describes the state of the field when they started, and tells about the beginnings of their collaboration.

It emphasizes the importance of various mentors in their lives, especially Stephen W. Kuffler, who opened up the field by studying the cat retina in 1950, and founded the department of neurobiology at Harvard Medical School, where most of their work was done. The main part of the book consists of Hubel and Wiesel’s most important publications. Each reprinted paper is preceded by a foreword that tells how they went about the research, what the difficulties and the pleasures were, and whether they felt a paper was important and why. Each is also followed by an afterword describing how the paper was received and what developments have occurred since its publication. The reader learns things that are often absent from typical scientific publications, including whether the work was difficult, fun, personally rewarding, exhilarating, or just plain tedious. The book ends with a summing-up of the authors’ view of the present state of the field. This is much more than a collection of reprinted papers. Above all it tells the story of an unusual scientific collaboration that was hugely enjoyable and served to transform an entire branch of neurobiology. It will appeal to neuroscientists, vision scientists, biologists, psychologists, physicists, historians of science, and to their students and trainees, at all levels from high school on, as well as anyone else who is interested in the scientific process.

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Radiation Safety in Nuclear Medicine, Second Edition

Recent advances in the field of nuclear medicine (NM) are expanding the role and responsibilities of the nuclear medicine technologist (NMT) to include more complex and detailed tasks. New technologies are making the diagnosis, management, and treatment of illnesses more sensitive, more specific, more accurate, and ultimately safer for both the patient and the technologist.
Radiation Safety in Nuclear Medicine, Second Edition provides the latest technological advances and expanded responsibilities of today’s NMT while laying a solid foundation for understanding the basic physics behind the technology. As with the original, this edition teaches the units of radioactivity, exposure, and dosimetry, along with the principles of instrumentation needed for detection and measurement. Focusing on the issues of safety, this volume devotes considerable attention to the science and practice of safety techniques and includes information on rules and regulations.

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Handbook of Neuro-Oncology Neuro-Imaging

Although the field of Neuro-Oncology has grown considerably in the last 10 to 15 years and has a rather extensive literature, there are no comprehensive, single-source books that summarize the current literature and future trends of neuroimaging in neuro-oncology. This book covers this topic in more comprehensive fashion, making it an important addition to the armamentarium of physicians that care for patients with brain tumors and other neuro-oncological disorders. Well-founded in basic science, it includes chapters that provide an overview of relevant background material in critical areas such as physics, contrast agents, ultra-high field brain MRI, and molecular imaging.


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Comprehensive Management of High Risk Cardiovascular Patients

Cardiovascular disease, including heart disease, peripheral arterial disease, and stroke, is the leading cause of illness and death in the United States. Filling a gap in the literature, this reference contains expert contributions on the care and management of patients at high-risk for cardiovascular disease and studies early indicators and risk factors that may impact therapeutic treatment, as well as aid in the early detection of heart conditions.

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Geriatric Diabetes

The number of elderly patients with diabetes is increasing at a significant rate. Responding to this growth, this source serves as a solid arsenal of information on the varying presentations and challenges associated with diabetes in the geriatric patient, and supplies clearly written sections on the screening, diagnosis, and treatment of diabetes in elderly populations. With contributions from renowned authorities from the national and international institutions, this reference will depict the role of specific co-morbidities, socioeconomic aspects, and caregiver issues on the effective management of diabetes in the older adult.

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Protein Engineering Protocols (Methods in Molecular Biology, Vol 352)

Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering.

The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented.

Following the successful Methods in Molecular Biologyâ„¢ series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators.

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The Spina Bifida: Management and Outcome

The evolution of technology has made new surgical techniques available for the treatment of diseases associated with the spina bifida malformation, and this is especially true in hydrocephalus and fetal surgery. The progress in this last field and in prenatal diagnosis have raised the significant problem of the effectiveness and usefulness of in utero surgical treatment of the malformation, that are extensively discussed in this volume. Also the clinical situations that can be seen in adolescents or young adults with spina bifida, including sexuality and pregnancy, are highlighted. Aim of this book is to promote a multidisciplinary approach to spina bifida, providing the three main specialists’ categories involved - neurosurgeons, orthopaedic surgeons, and urologists - with an updated overview of surgical approaches and with a concise reference that explains the main clinical problems to be faced in everyday clinical practice.

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Terminal Ballistics: A Text and Atlas of Gunshot Wounds

Terminal Ballistics: A Text and Atlas of Gunshot Wounds is a comprehensive guide that explains the science of forensic ballistics - focusing on the hardware, injury patterns, and forensic techniques associated with gunshot wounds. The text begins by describing the anatomy and design of basic firearms and the fundamental components of ammunition rounds, .22 rimfire, and propellants. The author presents a variety of gunshot injury patterns, and defines the concept of the pathological range of fire determination. He delineates patterns from injuries sustained by commonly fired rounds, including those acquired after discharge from handguns, high velocity centerfire rifles, shot guns, and black powder weapons.

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Cardiovascular Magnetic Resonance Imaging (Contemporary Cardiology)

Cardiovascular Magnetic Resonance Imaging (CMR) is a rapidly evolving tool for cardiovascular diagnosis, and is becoming increasingly important in guiding cardiovascular interventions. Cardiovascular Magnetic Resonance Imaging presents a state-of-the-art compilation of expert contributions to the field, each examining normal and pathologic anatomy of the cardiovascular system as assessed by magnetic resonance imaging. Functional techniques such as myocardial perfusion imaging and assessment of flow velocity are emphasized, along with the exciting areas of artherosclerosis plaque imaging and targeted magnetic resonance imaging. Cardiovascular Magnetic Resonance Imaging represents a multi-disciplinary approach to the field, with contributions from experts in cardiology, radiology, physics, engineering, physiology and biochemistry and offers new directions in noninvasive imaging.

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The Lopsided Ape: Evolution of the Generative Mind

What is it that allows human beings to think the way we do? What enables us to communicate with one another through the use of speech? Is the difference between Homo sapiens and other apes simply a matter of degree or are we unique and discontinuous from other species? Michael C. Corballis argues that this century-old debate lies in the fact that humans are the only primates that are predominantly right-handed, a sign of the specialization of the left hemisphere of the brain for language. He attributes humans’ unique abilities to a biological mechanism in the left hemisphere of the brain called a “generative learning device” or GAD. The GAD, Corballis contends, enables us to generate a limitless number of forms and meanings from a few parsed elements, providing the basis for language and manufacture as well as mathematics, reasoning, art, music, and play. Surveying the current views of evolution using evidence from archeology, linguistics, neurology, and genetics, Corballis takes us on a fascinating tour of the origins and implications of the structure of the human brain accounting for the dominance of humanity over all species.

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Membrane Structure in Disease and Drug Therapy

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This study asserts that cellular and intracellular membranes are active in every aspect of the body’s physiology and pathophysiology. It compares secondary through to quaternary structures and protien sequences and guages their influence on health, disease and drug therapy. The book highlights the importance of correlations, homologies and categorizing multifunctionality by domain and complex.

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